Journal: Oncotarget

Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity

doi: 10.18632/oncotarget.6488

Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Article Snippet: The normal human foreskin fibroblast cell line Hs68 was provided by JCRB Cell Bank (Osaka, Japan).

Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 3. 2D-PAGE image from human fibroblast(control).

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques: Control

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 4. 2D-PAGE image from human fibroblast. Cell treated with AX. Extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 2. Cell toxicity of fibroblast treated with AX extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: The cytotoxicity and effect on intracellular oxidative stress in Hs68 of TCE. a Cell viability (%) of human fibroblasts (Hs68), human keratinocytes, and mouse melanoma cells treated with TCE; b Cell viability of Hs68 after treatment with TCE with or without 150 μM hydrogen peroxide (H 2 O 2 ) exposure. c Repressive effect of TCE on H 2 O 2 -induced intracellular oxidative stress in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Control, Inhibition

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: Effect of TCE on the H 2 O 2 -induced ( a ) phosphorylation of mitogen-activated protein kinases, ( b ) c-Jun phosphorylation and c-Fos, ( c ) matrix metalloproteinase (MMP)-1, − 3, − 9, ( d ) hemeoxygenase-1 (HO-1), ( e ) cyclooxygenase-2 (COX-2) and ( f ) type I procollagen protein expressions in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01; ### p < 0.001. EGCG: (−)-epigallocatechin gallate was used as a positive control

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Phospho-proteomics, Control, Inhibition, Positive Control